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<h1>Safety</h1>
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<h1>Researcher safety</h1>
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<p>Our researchers mainly face regular bio-safety challenges in a
molecular microbiology lab, such as risks staining DNA agarose gel with
EB, extracting RNA from organisms with TRIZOL, and manipulating with
DEPC treated water, etc. Any researchers conducting such experiment<img
	src="http://2011.igem.org/wiki/images/4/42/ZJUsafety2.jpg" width="300"
	style="float: right;" /> are instructed about the right method both by
printed protocols and BIG BROTHER in the lab (XD). After the training,
we must pass an on-line test about the lab safety, standard experiment
procedure and lab waste treatment. During any experiments, necessary
individual protections such as gloves and white robes are always
required. Also, the public safety of the lab is guaranteed by the strict
registering protocols on the operation of any apparatus. In order to
make sure all the requirements above were followed well by the
researchers and to get prepared for any emergency, our lab (also is the
open bio-lab of the college) set up 24hr*7 camera system in the room.</p>
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<h1>Public safety</h1>
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<p>First we picked DH5α and JM109 as the two strains to manipulate
in our research. These are two non-pathogenic E. coli strain and do not
pose problems to the public health. The bio-parts we utilized are all
security ensured, coding for florescence protein, cellulose degradation
enzymes and metal ion binding proteins. Although bio-film formation is
closely related to pathogenicity, our experiment enhances bio-film
formation only by changing the external environment factors instead of
modifying the inherited nature of the bacteria. This is an important
measure to assure that the bacteria do not acquire previously
non-existing pathogenic capacity.</p>
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<h1>Environment safety</h1>
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<p>The experiments conducted in our lab are with intensive concern
of environmental impact. No media containing engineered germs go into
the sewer before sterilization. Besides, all the utensil contacted with
the engineered bacteria must first undergo sterilization then be washed.
During our research we have used antibiotic resistant plasmids,
including chloramphenicol, kanamycin, ampicillin. Though risks of the
practices exist, we believe that it's possible for us to use them safely
with the mature protocols and techniques. For the toxic and hazardous
chemicals, they are collected and treated properly. The acid and basic
chemicals are neutralized and pour into the swer.</p>
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<h1>Biobrick safety</h1>
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<p><img src="http://2011.igem.org/wiki/images/4/46/Zjusafety1.jpg"
	width="220" style="float: left;">Our biobricks includes two new
promoters responded to oxygen concentration, hence the regulation of the
devices would not involve potentially bio-hazard chemicals. Also as
partially described above, our parts express proteins aiming at binding
of metal ions, degradation of cellulose, and the localization by
fluorescent proteins. All of these products are not bio-hazard, and the
expression is under fine control.</p>
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<h1>Biosafety provisions</h1>
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<p>Our experiment is designed and carried out following the
standards of national biosafety office, especially the <a
	href="http://www.biosafety.gov.cn/gjzcfg/flfg/200401/t20040115_88044.htm"
	target="_blank">《Safety Administration Regulation on Genetic
Engineering》</a> (Only the chinese version is available online),as well as <a
	href="http://www.zjubiolab.zju.edu.cn/page/news.php?action=introlist&news_catalogId=33"
	target="_blank">the laboratory regulations</a> of our university, the
college of life sciences, and the biology lab center.</p>
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